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Assay Design for MIP Genotyping
Description:
For each SNP a single MIP probe is designed. A minimum of 5000 probes are designed as a probe set.
1) MIP probe design
Two homologies are designed that abut, but do not overlap the SNP. The tag sequence, two primer
sequences and a restriction sequence are added to homology 1 and homology 2 in the order (5' - 3')
homology 2, tag sequence, restriction site, primer P2, primer P1, and homology 1.
