西亞試劑：A complex network of factors with overlapping affinities re

A complex network of factors with overlapping affinities represses splicing through intronic elements

Yang Wang,1 Xinshu Xiao,2, 3 Jianming Zhang,2, 4 Rajarshi Choudhury,1 Alex Robertson,2 Kai Li,1 Meng Ma,1, 5 Christopher B Burge2 & Zefeng Wang1

To better understand splicing regulation, we used a cell-based screen to identify ten diverse motifs that inhibit splicing from introns. Motifs were validated in another human cell type and gene context, and their presence correlated with in vivo splicing changes. All motifs exhibited exonic splicing enhancer or silencer activity, and grouping these motifs according to their distributions yielded clusters with distinct patterns of context-dependent activity. Candidate regulatory factors associated with each motif were identified, to recover 24 known and new splicing regulators. Specific domains in selected factors were sufficient to confer intronic-splicing-silencer activity. Many factors bound multiple distinct motifs with similar affinity, and all motifs were recognized by multiple factors, which revealed a complex overlapping network of protein-RNA interactions. This arrangement enables individual cis elements to function differently in distinct cellular contexts, depending on the spectrum of regulatory factors present

• 以上資料由西亞試劑：http://hendrickstechnology.com/ 提供此產(chǎn)品的詳細(xì)信息如密度,含量,分子式,分子量等均可在西亞官網(wǎng)查詢   
• 相關(guān)產(chǎn)品如溴化汞硝酸亞汞氯化亞汞乙酸苯汞氯化鉀汞 碘化汞鉀硫氰酸汞硫酸亞汞氧化汞氯化汞碘化汞硝酸汞三氯氧磷三氯化磷碘甲烷二碘甲烷三碘甲烷三氟碘甲烷氘代碘甲烷碘乙烷1,2-二碘乙烷甲酸銣碘化銣溴化銣鉻酸銣硫酸銣氟化銣硝酸銣氯化銣碳酸銣硫酸鐿 碳酸鐿氯化鐿硝酸鐿氧化鐿等均有銷售.歡迎訂購