西亞試劑：Structure of a pathogenic type 3 secretion system in action

Structure of a pathogenic type 3 secretion system in action

Julia Radics，  Lisa Knigsmaier  & Thomas C Marlovits

Type 3 secretion systems use 3.5-megadalton syringe-like， membrane-embedded 'injectisomes'， each containing an ~800--long needle complex to connect intracellular compartments of infectious bacteria and hosts. Here we identify requirements for substrate association with， transport through and exit from the injectisome of Salmonella enterica serovar Typhimurium. This guided the design of substrates that become trapped within the secretion path and enabled visualization of injectisomes in action in situ. We used cryo-EM to define the secretion path， providing a structural explanation as to why effector proteins must be unfolded during transport. Furthermore， trapping of a heterologous substrate in the needle prevents secretion of natural bacterial effectors. Together， the data reveal the path of protein secretion across multiple membranes and show that mechanisms rejecting unacceptable substrates can be undermined， and transport of bacterial effectors across an already assembled type 3 secretion system can be inhibited

• 以上資料由西亞試劑：http://hendrickstechnology.com/ 提供此產(chǎn)品的詳細(xì)信息如密度,含量,分子式,分子量等均可在西亞官網(wǎng)查詢(xún)   
• 相關(guān)產(chǎn)品如溴化汞硝酸亞汞氯化亞汞乙酸苯汞氯化鉀汞 碘化汞鉀硫氰酸汞硫酸亞汞氧化汞氯化汞碘化汞硝酸汞三氯氧磷三氯化磷碘甲烷二碘甲烷三碘甲烷三氟碘甲烷氘代碘甲烷碘乙烷1,2-二碘乙烷甲酸銣碘化銣溴化銣鉻酸銣硫酸銣氟化銣硝酸銣氯化銣碳酸銣硫酸鐿 碳酸鐿氯化鐿硝酸鐿氧化鐿等均有銷(xiāo)售.歡迎訂購(gòu)