西亞試劑：Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-

Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos

Yuyu Niu， Bin Shen， Yiqiang Cui， Yongchang Chen， Jianying Wang， Lei Wang， Yu Kang， Xiaoyang Zhao， Wei Si， Wei Li， Andy Peng Xiang， Jiankui Zhou， Xuejiang Guo， Ye Bi， Chenyang Si， Bian Hu， Guoying Dong， Hong Wang， Zuomin Zhou， Tianqing Li， Tao Tan， Xiuqiong Pu， Fang Wang， Shaohui Ji， Qi Zhou， Xingxu Huang， Weizhi Ji， Jiahao Sha

Monkeys serve as important model species for studying human diseases and developing therapeutic strategies， yet the application of monkeys in biomedical researches has been significantly hindered by the difficulties in producing animals genetically modified at the desired target sites. Here， we first applied the CRISPR/Cas9 system， a versatile tool for editing the genes of different organisms， to target monkey genomes. By coinjection of Cas9 mRNA and sgRNAs into one-cell-stage embryos， we successfully achieve precise gene targeting in cynomolgus monkeys. We also show that this system enables simultaneous disruption of two target genes (Ppar-γ and Rag1) in one step， and no off-target mutagenesis was detected by comprehensive analysis. Thus， coinjection of one-cell-stage embryos with Cas9 mRNA and sgRNAs is an efficient and reliable approach for gene-modified cynomolgus monkey generation

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