西亞試劑：Structure of Gq-p63RhoGEF-RhoA Complex Reveals a Pathway fo

Structure of Gq-p63RhoGEF-RhoA Complex Reveals a Pathway for the Activation of RhoA by GPCRs

Susanne Lutz,1* Aruna Shankaranarayanan,2,3* Cassandra Coco,2 Marc Ridilla,2 Mark R. Nance,2 Christiane Vettel,1 Doris Baltus,1 Chris R. Evelyn,4 Richard R. Neubig,4 Thomas Wieland,1 John J. G. Tesmer2,4

The guanine nucleotide exchange factor p63RhoGEF is an effector of the heterotrimeric guanine nucleotide–binding protein (G protein) Gq and thereby links Gq-coupled receptors (GPCRs) to the activation of the small-molecular-weight G protein RhoA. We determined the crystal structure of the Gq-p63RhoGEF-RhoA complex, detailing the interactions of Gq with the Dbl and pleckstrin homology (DH and PH) domains of p63RhoGEF. These interactions involve the effector-binding site and the C-terminal region of Gq and appear to relieve autoinhibition of the catalytic DH domain by the PH domain. Trio, Duet, and p63RhoGEF are shown to constitute a family of Gq effectors that appear to activate RhoA both in vitro and in intact cells. We propose that this structure represents the crux of an ancient signal transduction pathway that is expected to be important in an array of physiological processes.

1 Institute of Experimental and Clinical Pharmacology and Toxicology, Medical Faculty Mannheim, University of Heidelberg, Maybachstrasse 14, D-68169 Mannheim, Germany.
2 Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109–2216, USA.
3 Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712–0165, USA.
4 Department of Pharmacology, University of Michigan, Ann Arbor, MI 48109–0632, USA.

* These authors contributed equally to this work.

 Present address: Department of Biological Sciences, Purdue University, 915 West State Street, West Lafayette, IN 47907, USA.

 To whom correspondence should be addressed. E-mail:

• 以上資料由西亞試劑：http://hendrickstechnology.com/ 提供此產(chǎn)品的詳細(xì)信息如密度,含量,分子式,分子量等均可在西亞官網(wǎng)查詢   
• 相關(guān)產(chǎn)品如溴化汞硝酸亞汞氯化亞汞乙酸苯汞氯化鉀汞 碘化汞鉀硫氰酸汞硫酸亞汞氧化汞氯化汞碘化汞硝酸汞三氯氧磷三氯化磷碘甲烷二碘甲烷三碘甲烷三氟碘甲烷氘代碘甲烷碘乙烷1,2-二碘乙烷甲酸銣碘化銣溴化銣鉻酸銣硫酸銣氟化銣硝酸銣氯化銣碳酸銣硫酸鐿 碳酸鐿氯化鐿硝酸鐿氧化鐿等均有銷售.歡迎訂購(gòu)